Product Watch

Stat Diagnostic Services
Urea breath test

Since Barry Marshall described the Helicobacter pylori (Hpylori) and its role in different gastrointestinal diseases, an intense research effort has developed different diagnostic methods. The only non-ivasive test method that can be clinically used as a diagnostic tool for on-going infection with H pylori and post-eradication follow-up is the ‘urea breath test’. Different serological tests are available, but they cannot determine the actual status of the infection and can therefore only be used for screening tests. With the Radprobe TM, a cost-effective test is available, easy to use in the doctor's office.

How does Heliprobe work?
The Urea Breath Test method (UBT)

Is based on the fact that the bacteria H pylori produces an enzyme, urease, which catalyes the hydrolysis of the urea molecule into ammonium and carbon dioxide. H plyori requires the alkaline ammonium in order to establish an optimal local mucosal environment within the acidic environment of the stomach. The urea compound is an endogenous substance that contains one carbon atom, normally C, which can be replaced by its isotope C or C. Heliprobe TM uses the isotope C which has very low B-radioactive emission. The radioactive dose used for a Heliprobe test is 1 uCurie, which is significantly less than the radioactive dose given at a normal X-ray examination of the stomach, and equal to a few glasses of orange juice.

A Helicap is ingested with 50 ml of water. If an infection with H pylori is present in the stomach, the ‘living’ bacteria produces the enzyme ‘urease’ that metabolises the C-urea into ammonium and carbon dioxide containing the C-labelled atoms. The carbon dioxide will be assimilated through the mucosa into the blood stream and transported to the lungs where it is exhaled. By collecting the exhaled carbon dioxide in the BreathCard, the amount of Co2 produced in the stomach can be measured with the Heliprobe TM instrument. If the patient is not infected, no metabolisation of urea will occur and therefore no CO2 can be detected with the Heliprobe system. In this case, the unmetabolised C urea will pass along the digestive canal to be excreted in the urine. If the Heliprobe system detects Co2, this is diagnostic evidence of an ongoing infection with living H pylori. Heliprobe is developed for primary diagnosis of H pylori, as well as for follow-up after eradication treatment.

Laboratory and Clinical Evaluation

To ensure equivalent results comparable with sophisticated, well-established laboratory techniques used for scientific purposes, the following comparison between Heliprobe TM and liquid scintillation for C detection was made. A laboratory set-up (simulating the patient) with controlled parameters was established. The result of the comparison was statistically analysed applying linear regression. The outcome of the analysis shows a regression line with high correlation between these methods. Clinical validation studies involving more than 400 patients has also been performed at the Karolinska University Hospital in Stockholm, Sweden.

Contact:
Stat Diagnostics Services
3rd Floor, Chandrapushpa Building
Opp. New Post Office
Hirachand Desai Marg
Ghatkopar (West)
Mumbai-86
Tel: 91-22-25112424
91-22-25157206

Schiller Healthcare India Pvt Ltd
Defigard 5000 Hospital Defibrillator

Schiller India's hospital defibrillator—Defigard 5000 has been doing extremely well in the hospital market. Schiller has successfully installed it in many leading hospitals such as Parsee General Hospital, Holy Family Hospital, GB Pant Hospital, AIIMS, Banaras Hindu University (BHU) and LNJP Hospital. According to Hormazd Cooper, Vice President, Sales, "The Defigard 5000 is a high-end defibrillator targeted specially at hospitals. This unit offers the industry's largest colour screen for optimum viewing of all parameters. Using Schiller's patented multi-pulse biowave and biphasic technology, guarantees lower tissue damage. The unit also offers physicians fixed demand and over-ride modes of pacing and uses the same electrode set which is used for normal defibrillation, thereby ensuring economical operation."

Features

• Large 10.4" colour TFT screen with manual and AED modes for adult and paediatric application.
• User friendly operation by audible and visual instructions and guidance on screen.
• Capability to include monitoring parameters such as ECG, SPO2 and NIBP.
• Integrated three channel printer with auto and manual modes.
• Display of AED prompts, measurements and waveforms, of ECG, SPO2 and NIBP.
• Internal memory for 45 minutes of ECG and 500 events in AED as well as 24 hour trends (graphical and tabular) for all parameters.
• Integrated three-channel printer.
• Automatic and manual printing for all waveforms, measurements, events, trends, and alarms.
• Internal defibrillation with internal spoon electrode (optional).
• Download of AED data via USB port using memory stick (optional).
• Transmission of 12-channel ECG via GSM or modem with ethernet connection for software upgrades (optional).

Contact:
Hansa Soneji
Product Specialist
Schiller Healthcare India Pvt Ltd
DC Silk Mills Compound, Kondivitta Lane
Andheri East
Mumbai 400059
Tel: (022) 66920520
Fax: (022) 28263525
E-mail: hansa@schillerindia.com

Bio-Rad Laboratories India Pvt Ltd
ProteoMiner Protein Enrichment Kits

High-abundance proteins present in complex biological samples such as sera or plasma make the detection of medium and low-abundance proteins extremely challenging. ProteoMiner protein enrichment technology is a novel sample preparation tool used to reduce the dynamic range of proteomes from complex biological samples, allowing the exploration of the entire proteome.

ProteoMiner protein enrichment technology employs a large, highly diverse bead-based library of combinatorial peptide ligands, which simultaneously reduces high-abundance proteins and enriches low-abundance proteins. When a complex biological sample is applied to the beads, high-abundance proteins saturate their high-affinity ligand or ligands, and excess protein is washed away. In contrast, low-abundance proteins are concentrated on their specific affinity ligand or ligands.

Each bead features a different hexapeptide ligand with affinity for specific proteins in a sample. Samples are applied to the beads, allowing proteins to bind to their specific ligands. Proteins in excess are washed away, and those proteins bound to the beads are eventually eluted, allowing further downstream analysis.

Benefits

• Decreases the amount of high-abundance proteins without immunodepletion-prevents the co-depletion of proteins bound to high-abundance proteins.
• Enriches medium and low-abundance proteins that cannot be detected through traditional methods.
• Compatible with a variety of sample types, independent of species specificity.
• Not dependent on a predefined set of antibodies, unlike immunodepletion products.
• Compatible with all major downstream protein analysis techniques.
• Can be used for differential expression analysis.

Convenient, easy-to-use format

• Spin column format is easy to use.
• One-time use, avoids potential for contamination between samples.
• All necessary columns and reagents provided in kits.

Contact:
Bio-Rad Laboratories India Pvt Ltd
Bio-Rad House
86-87, Udyog Vihar, Phase - IV, Gurgaon - 122 015
Haryana
Phone: 91-124-4029300
Fax: 91-124-2398115